Before preforming you MD simulation, proteins should be solvated into a water box. Try not to run MD simulation in vacuum these days, update you computer or just apply for some computational resources to Nankai Stars. ^_^
If you use gromacs, you can use genion to add ions to you system. Actually, genion is quite stupid. It just place the ions around the protein, because it thinks that is the low potential energy solution. you can use the -random flag to place the added ions all around the system. if you find out some ions are too close to the protein, change the -seed flag until you think it is reasonable.
Amber suite have two commands to add ions into MD system: addions and addions2. Both of them have some drawbacks. addions just add ions around the protein, while addions2 add ions to the system at the edge of the bound box. I think addions2 is more reasonable, cause it does not cause some artificial effects. But if you add some positive ions and some negative ions at the same time using addions2, both positive and negative ions will be at the edge of the bound box, then after the energy minimization, they will bound to each other. that is too bad.
Too solve the addions/addions2 problems in amber suite, you can use addions to add some ions at first, and use addions2 at the second time to neutralize the system. Or you can solvate your protein a little bit, then use addions2 to add some ions, then fully solvate your system and use addions2 to neutralize the whole system.
And how about Namd? you can tell me. :D
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